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- will be discontinued soon
Polyclonal antibodies are a finite resource. We have now started to sell the last lots of our well received Guinea pig polyclonal Synaptoporin antibody 102 005. It will be replaced by our new Guinea pig monoclonal recombinant IgG Synaptoporin antibody 102 308.

Synaptoporin (p38-2) antibody - 102 005

Synaptoporin s a synaptic vesicle membrane protein similar to synaptophysin1 enriched in Mossy fibres
Guinea pig polyclonal purified antibody
Cat. No.: 102 005
Amount: 50 µg
Price: $465.00
Cat. No. 102 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 250 to 265 from rat Synaptoporin (UniProt Id: P22831)
Reactivity Reacts with: rat (P22831), mouse (Q8BGN8), hamster.
Other species not tested yet.
Matching control protein/peptide 102-0P
Remarks

WB: Less powerful compared to the rabbit antibodies (102 002 and 102 003).

Data sheet 102_005.pdf
Cat. No.: 102 005
Amount: 50 µg
Price: $465.00
Background

Synaptoporin, also known as synaptophysin2 and p38-2, is highly homologous to synaptophysin1 but encoded by a different gene. Like synaptopysin1, synaptoporin contains four transmembrane regions and a short cytoplasmic tail. Unlike synaptophysin1, it is not glycosylated.
The distributions of synaptophysin1 and synaptoporin are different. Synaptophysin1 is more uniformly expressed whereas synaptoporin is particularly enriched in mossy fiber synapses in the hippocampus. It is thus an excellent marker for subsets of synapses.