The ALFA Selector resins can be eluted by different methods.
Peptide elution under native conditions (ALFA SelectorPE only)
This elution mode is based on competition between the ALFA elution peptide present in the elution buffer and the ALFA-tagged target protein for available binding sites on the resin. To obtain convenient elution kinetics, peptide elution has to be performed at room temperature (22 - 25°C).
Acidic elution (ALFA SelectorPE / ALFA SelectorST)
ALFA-tagged target proteins can efficiently be eluted from both ALFA Selector variants at low pH with Acidic elution buffer.
Denaturing elution using SDS sample buffer (ALFA SelectorPE / ALFA SelectorST)
ALFA-tagged target proteins can be efficiently eluted from both ALFA Selector variants using SDS sample buffer at elevated temperatures.