anti-Rabbit IgG sdAb - N2405-DBCO

A monovalent single domain secondary antibody specific for rabbit IgG

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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N2405-DBCO

Amount: 250 µg

Price: $770.00

Cat. No. N2405-DBCO	250 µg purified antibody, lyophilized from PBS, fluorescence-labeled with DBCO. Dibenzocyclooctyne (DBCO, ADIBO) is one of the most reactive cycloalkynes for strain promoted alkyne azide cycloaddition (spAAC), a copper-free click chemistry reaction. Albumin was added for stabilization. For reconstitution refer to the NanoTag reconstitution and storage instructions. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	ICC: yes IHC: yes FACS: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Label	DBCO
Clone	10E10
Reactivity	Reacts with: rabbit. No signal: chicken, mouse, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet.
Specificity	Rabbit IgG, does not cross-react with mouse, guinea pig or chicken immunoglobulins

Cat. No.: N2405-DBCO

Amount: 250 µg

Price: $770.00

Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

FluoTag®-X anti-Rabbit is a species-specific FluoTag® directed against the IgG from Rabbit. In FluoTag-X2, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Rabbit antibody. FluoTag-X4 is a blend of two nanobodies carrying two fluorophores each. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since FluoTags are recombinantly expressed, there is no batch-to-batch variation.

Protocols

ICC

IHC

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