ALFA antibody super-tight - N1582

The ALFA tag is a novel peptide tag

This product was developed by

The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts. Different ALFA-tag based products like FluoTags, polyclonal and monoclonal antibodies and Selector resins are available.

Mouse monoclonal recombinant IgG

Cat. No.: N1582

Amount: 100 µg

Price: $515.00

Cat. No. N1582	100 µg purified recombinant IgG, lyophilized. Albumin was added for stabilization. For reconstitution refer to the NanoTag reconstitution and storage instructions. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	1G5
Subtype	IgG1
Immunogen	Synthetic peptide corresponding to: SRLEEELRRRLTE (UniProt Id: not defined)
Specificity	Specific for ALFA-tag
Remarks	This antibody is a chimeric construct consisting of the anti-ALFA single-domain antibody 1G5 genetically fused to mouse IgG1 Fc domain. It can be used in combination with standard anti-mouse secondary reagents binding the IgG Fc-domain.

References for ALFA - N1582

A light-controlled phospholipase C for imaging of lipid dynamics and controlling neural plasticity.
Kim YJ, Tohyama S, Nagashima T, Nagase M, Hida Y, Hamada S, Watabe AM, Ohtsuka T
Cell chemical biology (2024) : . N1582 WB; tested species: mouse,human

Cat. No.: N1582

Amount: 100 µg

Price: $515.00

ICC

Background

The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts.
The ALFA-tag has the broadest spectrum of applications in life sciences compared to any other tag.

Protocols

ICC

IHC

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