ALFA antibody - N1580

The ALFA tag is a novel peptide tag

This product was developed by

The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts. Different ALFA-tag based products like FluoTags, polyclonal and monoclonal antibodies and Selector resins are available.

Rabbit polyclonal antiserum

Cat. No.: N1580

Amount: 200 µl

Price: $515.00

Cat. No. N1580	200 µl antiserum, lyophilized. For reconstitution refer to the NanoTag reconstitution and storage instructions. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (ECL detection) gallery IP: not tested yet ICC: 1 : 500 IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to: SRLEEELRRRLTE (UniProt Id: not defined)
Specificity	Specific for ALFA-tag

Cat. No.: N1580

Amount: 200 µl

Price: $515.00

Background

The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts.
The ALFA-tag has the broadest spectrum of applications in life sciences compared to any other tag.

Protocols

ICC

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