Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home | | | | | Technical Support
Home > Products > N1202-At488-S

anti-Mouse IgG kappa light chain sdAb - FluoTag-X2 - N1202-At488-S

Secondary single domain antibody specific for mouse IgG with a kappa light chain

 

This product was developed by   

 NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N1202-At488-S
Amount: 500 µl
Price: $250.00
Cat. No. N1202-At488-S 500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488.

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established fluorescence detection systems can be used.

ATTO® 488: λex 500 nm / λem 520 nm
ATTO® 550: λex 554 nm / λem 576 nm
ATTO® 565: λex 564 nm / λem 590 nm
ATTO® 594: λex 603 nm / λem 626 nm
ATTO® 647N: λex 646 nm / λem 664 nm
 

ATTO®488, ATTO®565, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®488, ATTO®565 and ATTO®594 are also recommended for PALM and dSTORM high resolution microscopy.

This product or portions thereof is manufactured under license from ATTO-TEC GmbH.
ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH.
Albumin was added for stabilization. For reconstitution refer to the NanoTag reconstitution and storage instructions.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 1A23
Subtype single domain
Reactivity Reacts with: mouse.
No signal: rat, rabbit, goat, Guinea pig, donkey, human, cow, horse, pig.
Other species not tested yet.
Specificity Recognizes all mouse IgG subtypes that contain a κ light chain (most do).
It does not cross-react to rat, rabbit, chicken, Guinea pig, or goat IgGs.

References for anti-Mouse IgG kappa light chain sdAb - N1202-At488-S

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S, et al.
Nature communications (2019) 101: 4403. N1202-At488-S IP

Cat. No.: N1202-At488-S
Amount: 500 µl
Price: $250.00
The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S, et al.
Nature communications (2019) 101: 4403. N1202-At488-S IP
ICC
Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

FluoTag®-X2 anti-Mouse is a species-specific FluoTag® directed against the κ light chain of mouse.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary mouse antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.
Other products for this target