anti-Chicken IgY sdAb - FluoTag-X2 - N0702-At488-S

Secondary single domain antibody specific for chicken IgY

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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N0702-At488-S

Amount: 500 µl

Price: $250.00

Cat. No. N0702-At488-S	500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO^® 488. For many of the fluorescence labeled antibodies conjugated to ATTO^® dyes from ATTO-TEC the established fluorescence detection systems can be used. ATTO^® 488: λ_ex 500 nm / λ_em 520 nm ATTO^® 550: λ_ex 554 nm / λ_em 576 nm ATTO^® 565: λ_ex 564 nm / λ_em 590 nm ATTO^® 594: λ_ex 603 nm / λ_em 626 nm ATTO^® 647N: λ_ex 646 nm / λ_em 664 nm ATTO^®488, ATTO^®565, ATTO^®647N and ATTO^®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. ATTO^®488, ATTO^®565 and ATTO^®594 are also recommended for PALM and dSTORM high resolution microscopy. This product or portions thereof is manufactured under license from ATTO-TEC GmbH. ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany. Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH. Albumin was added for stabilization. For reconstitution refer to the NanoTag reconstitution and storage instructions. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: yes ICC: 1 : 500 IHC: 1 : 500 Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Label	ATTO 488, two fluorophores coupled to one FluoTag
Clone	4B1
Subtype	single domain
Reactivity	Reacts with: chicken. No signal: mouse, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet.
Specificity	Recognizes native chicken IgY. It does not cross-react to mouse, rabbit, Guinea pig, rat, donkey, goat, pig, horse, cow or human IgGs.

Cat. No.: N0702-At488-S

Amount: 500 µl

Price: $250.00

Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

IgY is the major immunoglobulin found in chicken eggs. FluoTag^®-X2 anti-chicken is a species-specific FluoTag^®-X2 directed against immunoglobulin IgY from chicken.
In FluoTag^®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag^® molecule. Therefore, the reagent targets four fluorophores to your primary chicken antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization.

Protocols

ICC

IHC

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