Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home | | | | | Technical Support
Home > Products > N0702-AF647-S

anti-Chicken IgY sdAb - FluoTag-X2 - N0702-AF647-S

Secondary single domain antibody specific for chicken IgY

 

This product was developed by   

 NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N0702-AF647-S
Amount: 500 µl
Price: $250.00
Cat. No. N0702-AF647-S 500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with Alexa 647.

Fluorescence labeled antibodies conjugated to Alexa® dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

AZdye® 568: λex 568 nm / λem 665 nm (identical to Alexa® 568)
Alexa® 647: λex 650 nm / λem 665 nm

This product or portions thereof is manufactured under license from Life Technologies Corporation. 
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Purchase of reagents related to the Alexa Fluor technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from Life Technologies Corporation.
Albumin was added for stabilization. For reconstitution refer to the NanoTag reconstitution and storage instructions.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: yes
ICC: 1 : 500
IHC: 1 : 500
FACS: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Label Alexa 647, two fluorophores coupled to one FluoTag
Clone 4B1
Subtype single domain
Reactivity Reacts with: chicken.
No signal: mouse, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
Specificity Chicken IgY, does not cross-react with mouse, rabbit, Guinea pig or rat immunglobulins

Cat. No.: N0702-AF647-S
Amount: 500 µl
Price: $250.00
Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

IgY is the major immunoglobulin found in chicken eggs. FluoTag®-X2 anti-chicken is a species-specific FluoTag®-X2 directed against immunoglobulin IgY from chicken.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary chicken antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization.
Other products for this target