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RFP (mCherry) antibody - 390 004

RFP is a red fluorescent protein tag
Guinea pig polyclonal antiserum
Cat. No.: 390 004
Amount: 100 µl
Price: $375.00
Cat. No. 390 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 200 gallery  
iDISCO: external data (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

iDISCO is a fast and cost-effective sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. The clearing is carried out by benzyl-ether treatment. For additional experimental details, please refer to the „Remarks“ section or visit https://idisco.info.
Immunogen Recombinant protein corresponding to AA 1 to 236 from mCherry (UniProt Id: X5DSL3)
Specificity Recognizes mRFP, mCherry, mOrgange2, dsRed, tdTomato, mScarlet.
Remarks

iDISCO: This antibody has been successfully used for iDISCO according to del Mar Sánchez-Martín et al.
Data sheet 390_004.pdf

References for RFP - 390 004

Chromatin modification abnormalities by CHD7 and KMT2C loss promote medulloblastoma progression.
Wang W, Kumegawa K, Chapman OS, Shiraishi R, Xiao Z, Okonechnikov K, Sun Y, Pfister SM, Feng W, Uesaka N, Hoshino M, et al.
Cell reports (2025) : 115673. 390 004 ICC; tested species: mouse
Fetal rat neural progenitor cell transplantation after spinal cord injury improves motor recovery following optogenetic stimulation.
Sánchez-Martín MDM, Giraldo E, Alastrue-Agudo A, López-Mocholi E, Martín-Pérez S, Maninno L, Paniagua Soriano G, Fraga Sánchez AI, Monreal-Trigo J, Terrés-Haro JM, Vidal Beneyto Q, et al.
Molecular therapy : the journal of the American Society of Gene Therapy (2025) : . 390 004 ICC, IHC, iDISCO; tested species: rat
Hetero-pentamerization determines mobility and conductance of Glycine receptor α3 splice variants.
Lemmens V, Thevelein B, Vella Y, Kankowski S, Leonhard J, Mizuno H, Rocha S, Brône B, Meier JC, Hendrix J
Cellular and molecular life sciences : CMLS (2022) 7911: 540. 390 004 ICC
Impaired dynamic interaction of axonal endoplasmic reticulum and ribosomes contributes to defective stimulus-response in spinal muscular atrophy.
Deng C, Reinhard S, Hennlein L, Eilts J, Sachs S, Doose S, Jablonka S, Sauer M, Moradi M, Sendtner M
Translational neurodegeneration (2022) 111: 31. 390 004 ICC; tested species: mouse
Involvement of CaV 2.2 channels and α2 δ-1 in homeostatic synaptic plasticity in cultured hippocampal neurons.
Pilch KS, Ramgoolam KH, Dolphin AC
The Journal of physiology (2022) : . 390 004 ICC; tested species: mouse
Donor cell memory confers a metastable state of directly converted cells.
Kim KP, Li C, Bunina D, Jeong HW, Ghelman J, Yoon J, Shin B, Park H, Han DW, Zaugg JB, Kim J, et al.
Cell stem cell (2021) 287: 1291-1306.e10. 390 004 ICC; tested species: mouse
Cadherin 4 assembles a family of color-preferring retinal circuits that respond to light offset.
Olguin AGR, Rochon PL, Theriault C, Brown T, Yao H, Cayouette M, Cook EP, Krishnaswamy A
Current biology : CB (2025) 356: 1298-1310.e7. 390 004 IHC; tested species: mouse
Fetal rat neural progenitor cell transplantation after spinal cord injury improves motor recovery following optogenetic stimulation.
Sánchez-Martín MDM, Giraldo E, Alastrue-Agudo A, López-Mocholi E, Martín-Pérez S, Maninno L, Paniagua Soriano G, Fraga Sánchez AI, Monreal-Trigo J, Terrés-Haro JM, Vidal Beneyto Q, et al.
Molecular therapy : the journal of the American Society of Gene Therapy (2025) : . 390 004 ICC, IHC, iDISCO; tested species: rat
Stimulation of corticospinal neurons by optogenetic cAMP inductions promotes motor recovery after spinal cord injury in female rats via raphespinal tract modulation.
Martínez-Rojas B, Martín-Pérez S, Giraldo E, Lopez-Mocholi E, Alastrue A, Andrade-Talavera Y, Prius-Mengual J, Paniagua G, Pedraza M, Hingorani S, Rost BR, et al.
Nature communications (2025) 161: 5885. 390 004 IHC; tested species: rat
Cadherins orchestrate specific patterns of perisomatic inhibition onto distinct pyramidal cell populations.
Jézéquel J, Condomitti G, Kroon T, Hamid F, Sanalidou S, Garcés T, Maeso P, Balia M, Hu Z, Sahara S, Rico B, et al.
Nature communications (2025) 161: 4481. 390 004 IHC; tested species: mouse
Compartmentalized dendritic plasticity in the mouse retrosplenial cortex links contextual memories formed close in time.
Sehgal M, Filho DA, Kastellakis G, Kim S, Lee J, Shen Y, Huang S, Lavi A, Fernandes G, Davila Mejia I, Martin SS, et al.
Nature neuroscience (2025) : . 390 004 IHC; tested species: mouse
Modulation of stress-related behaviour by preproglucagon neurons and hypothalamic projections to the nucleus of the solitary tract.
Holt MK, Valderrama N, Polanco MJ, Hayter I, Badenoch EG, Trapp S, Rinaman L
Molecular metabolism (2024) 91: 102076. 390 004 IHC; tested species: mouse
Engram reactivation mimics cellular signatures of fear.
Suthard RL, Senne RA, Buzharsky MD, Diep AH, Pyo AY, Ramirez S
Cell reports (2024) 433: 113850. 390 004 IHC; tested species: mouse
Prelimbic cortex ensembles promote appetitive learning-associated behavior.
Surets M, Caban-Murillo A, Ramirez S
Learning & memory (Cold Spring Harbor, N.Y.) (2024) 311-2: . 390 004 IHC; tested species: mouse
Optogenetic estimation of synaptic connections in brain slices.
Kashima T, Sasaki T, Ikegaya Y
Journal of neuroscience methods (2024) 412: 110298. 390 004 IHC; tested species: mouse
Populations of Hindbrain Glucagon-Like Peptide 1 (GLP1) Neurons That Innervate the Hypothalamic PVH, Thalamic PVT, or Limbic Forebrain BST Have Axon Collaterals That Reach All Central Regions Innervated by GLP1 Neurons.
Randolph AB, Zheng H, Rinaman L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 4431: . 390 004 IHC; tested species: rat
Transplantation of dorsal root ganglia overexpressing the NaChBac sodium channel improves locomotion after complete SCI.
Hingorani S, Paniagua Soriano G, Sánchez Huertas C, Villalba Riquelme EM, López Mocholi E, Martínez Rojas B, Alastrué Agudo A, Dupraz S, Ferrer Montiel AV, Moreno Manzano V
Molecular therapy : the journal of the American Society of Gene Therapy (2024) : . 390 004 IHC; tested species: rat
Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome.
Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, et al.
Neuron (2024) 1126: 942-958.e13. 390 004 IHC; tested species: drosophila
Crym-positive striatal astrocytes gate perseverative behaviour.
Ollivier M, Soto JS, Linker KE, Moye SL, Jami-Alahmadi Y, Jones AE, Divakaruni AS, Kawaguchi R, Wohlschlegel JA, Khakh BS
Nature (2024) : . 390 004 IHC; tested species: mouse
Generation of Dopamine Transporter (DAT)-mCherry Knock-in Rats by CRISPR-Cas9 Genome Editing.
Matsumoto N, Miyano M, Abe T, Kashima T, Kato-Ishikura E, Inoue KI, Liu J, Kiyonari H, Takeuchi H, Ikegaya Y
Biological & pharmaceutical bulletin (2024) 472: 394-398. 390 004 IHC; tested species: rat
Astrocyte-neuron subproteomes and obsessive-compulsive disorder mechanisms.
Soto JS, Jami-Alahmadi Y, Chacon J, Moye SL, Diaz-Castro B, Wohlschlegel JA, Khakh BS
Nature (2023) : . 390 004 IHC; tested species: mouse
A Cre-driver rat model for anatomical and functional analysis of glucagon (Gcg)-expressing cells in the brain and periphery.
Zheng H, López-Ferreras L, Krieger JP, Fasul S, Cea Salazar V, Valderrama Pena N, Skibicka KP, Rinaman L
Molecular metabolism (2022) 66: 101631. 390 004 IHC; tested species: mouse
Amygdala inhibitory neurons as loci for translation in emotional memories.
Shrestha P, Shan Z, Mamcarz M, Ruiz KSA, Zerihoun AT, Juan CY, Herrero-Vidal PM, Pelletier J, Heintz N, Klann E
Nature (2020) 5867829: 407-411. 390 004 IHC; tested species: mouse
Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome.
Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, et al.
bioRxiv : the preprint server for biology (2023) : . 390 004 IHC-G; tested species: drosophila
Fetal rat neural progenitor cell transplantation after spinal cord injury improves motor recovery following optogenetic stimulation.
Sánchez-Martín MDM, Giraldo E, Alastrue-Agudo A, López-Mocholi E, Martín-Pérez S, Maninno L, Paniagua Soriano G, Fraga Sánchez AI, Monreal-Trigo J, Terrés-Haro JM, Vidal Beneyto Q, et al.
Molecular therapy : the journal of the American Society of Gene Therapy (2025) : . 390 004 ICC, IHC, iDISCO; tested species: rat
Cat. No.: 390 004
Amount: 100 µl
Price: $375.00
Chromatin modification abnormalities by CHD7 and KMT2C loss promote medulloblastoma progression.
Wang W, Kumegawa K, Chapman OS, Shiraishi R, Xiao Z, Okonechnikov K, Sun Y, Pfister SM, Feng W, Uesaka N, Hoshino M, et al.
Cell reports (2025) : 115673. 390 004 ICC; tested species: mouse
Fetal rat neural progenitor cell transplantation after spinal cord injury improves motor recovery following optogenetic stimulation.
Sánchez-Martín MDM, Giraldo E, Alastrue-Agudo A, López-Mocholi E, Martín-Pérez S, Maninno L, Paniagua Soriano G, Fraga Sánchez AI, Monreal-Trigo J, Terrés-Haro JM, Vidal Beneyto Q, et al.
Molecular therapy : the journal of the American Society of Gene Therapy (2025) : . 390 004 ICC, IHC, iDISCO; tested species: rat
Hetero-pentamerization determines mobility and conductance of Glycine receptor α3 splice variants.
Lemmens V, Thevelein B, Vella Y, Kankowski S, Leonhard J, Mizuno H, Rocha S, Brône B, Meier JC, Hendrix J
Cellular and molecular life sciences : CMLS (2022) 7911: 540. 390 004 ICC
Impaired dynamic interaction of axonal endoplasmic reticulum and ribosomes contributes to defective stimulus-response in spinal muscular atrophy.
Deng C, Reinhard S, Hennlein L, Eilts J, Sachs S, Doose S, Jablonka S, Sauer M, Moradi M, Sendtner M
Translational neurodegeneration (2022) 111: 31. 390 004 ICC; tested species: mouse
Involvement of CaV 2.2 channels and α2 δ-1 in homeostatic synaptic plasticity in cultured hippocampal neurons.
Pilch KS, Ramgoolam KH, Dolphin AC
The Journal of physiology (2022) : . 390 004 ICC; tested species: mouse
Donor cell memory confers a metastable state of directly converted cells.
Kim KP, Li C, Bunina D, Jeong HW, Ghelman J, Yoon J, Shin B, Park H, Han DW, Zaugg JB, Kim J, et al.
Cell stem cell (2021) 287: 1291-1306.e10. 390 004 ICC; tested species: mouse
Cadherin 4 assembles a family of color-preferring retinal circuits that respond to light offset.
Olguin AGR, Rochon PL, Theriault C, Brown T, Yao H, Cayouette M, Cook EP, Krishnaswamy A
Current biology : CB (2025) 356: 1298-1310.e7. 390 004 IHC; tested species: mouse
Fetal rat neural progenitor cell transplantation after spinal cord injury improves motor recovery following optogenetic stimulation.
Sánchez-Martín MDM, Giraldo E, Alastrue-Agudo A, López-Mocholi E, Martín-Pérez S, Maninno L, Paniagua Soriano G, Fraga Sánchez AI, Monreal-Trigo J, Terrés-Haro JM, Vidal Beneyto Q, et al.
Molecular therapy : the journal of the American Society of Gene Therapy (2025) : . 390 004 ICC, IHC, iDISCO; tested species: rat
Stimulation of corticospinal neurons by optogenetic cAMP inductions promotes motor recovery after spinal cord injury in female rats via raphespinal tract modulation.
Martínez-Rojas B, Martín-Pérez S, Giraldo E, Lopez-Mocholi E, Alastrue A, Andrade-Talavera Y, Prius-Mengual J, Paniagua G, Pedraza M, Hingorani S, Rost BR, et al.
Nature communications (2025) 161: 5885. 390 004 IHC; tested species: rat
Cadherins orchestrate specific patterns of perisomatic inhibition onto distinct pyramidal cell populations.
Jézéquel J, Condomitti G, Kroon T, Hamid F, Sanalidou S, Garcés T, Maeso P, Balia M, Hu Z, Sahara S, Rico B, et al.
Nature communications (2025) 161: 4481. 390 004 IHC; tested species: mouse
Compartmentalized dendritic plasticity in the mouse retrosplenial cortex links contextual memories formed close in time.
Sehgal M, Filho DA, Kastellakis G, Kim S, Lee J, Shen Y, Huang S, Lavi A, Fernandes G, Davila Mejia I, Martin SS, et al.
Nature neuroscience (2025) : . 390 004 IHC; tested species: mouse
Modulation of stress-related behaviour by preproglucagon neurons and hypothalamic projections to the nucleus of the solitary tract.
Holt MK, Valderrama N, Polanco MJ, Hayter I, Badenoch EG, Trapp S, Rinaman L
Molecular metabolism (2024) 91: 102076. 390 004 IHC; tested species: mouse
Engram reactivation mimics cellular signatures of fear.
Suthard RL, Senne RA, Buzharsky MD, Diep AH, Pyo AY, Ramirez S
Cell reports (2024) 433: 113850. 390 004 IHC; tested species: mouse
Prelimbic cortex ensembles promote appetitive learning-associated behavior.
Surets M, Caban-Murillo A, Ramirez S
Learning & memory (Cold Spring Harbor, N.Y.) (2024) 311-2: . 390 004 IHC; tested species: mouse
Optogenetic estimation of synaptic connections in brain slices.
Kashima T, Sasaki T, Ikegaya Y
Journal of neuroscience methods (2024) 412: 110298. 390 004 IHC; tested species: mouse
Populations of Hindbrain Glucagon-Like Peptide 1 (GLP1) Neurons That Innervate the Hypothalamic PVH, Thalamic PVT, or Limbic Forebrain BST Have Axon Collaterals That Reach All Central Regions Innervated by GLP1 Neurons.
Randolph AB, Zheng H, Rinaman L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 4431: . 390 004 IHC; tested species: rat
Transplantation of dorsal root ganglia overexpressing the NaChBac sodium channel improves locomotion after complete SCI.
Hingorani S, Paniagua Soriano G, Sánchez Huertas C, Villalba Riquelme EM, López Mocholi E, Martínez Rojas B, Alastrué Agudo A, Dupraz S, Ferrer Montiel AV, Moreno Manzano V
Molecular therapy : the journal of the American Society of Gene Therapy (2024) : . 390 004 IHC; tested species: rat
Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome.
Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, et al.
Neuron (2024) 1126: 942-958.e13. 390 004 IHC; tested species: drosophila
Crym-positive striatal astrocytes gate perseverative behaviour.
Ollivier M, Soto JS, Linker KE, Moye SL, Jami-Alahmadi Y, Jones AE, Divakaruni AS, Kawaguchi R, Wohlschlegel JA, Khakh BS
Nature (2024) : . 390 004 IHC; tested species: mouse
Generation of Dopamine Transporter (DAT)-mCherry Knock-in Rats by CRISPR-Cas9 Genome Editing.
Matsumoto N, Miyano M, Abe T, Kashima T, Kato-Ishikura E, Inoue KI, Liu J, Kiyonari H, Takeuchi H, Ikegaya Y
Biological & pharmaceutical bulletin (2024) 472: 394-398. 390 004 IHC; tested species: rat
Astrocyte-neuron subproteomes and obsessive-compulsive disorder mechanisms.
Soto JS, Jami-Alahmadi Y, Chacon J, Moye SL, Diaz-Castro B, Wohlschlegel JA, Khakh BS
Nature (2023) : . 390 004 IHC; tested species: mouse
A Cre-driver rat model for anatomical and functional analysis of glucagon (Gcg)-expressing cells in the brain and periphery.
Zheng H, López-Ferreras L, Krieger JP, Fasul S, Cea Salazar V, Valderrama Pena N, Skibicka KP, Rinaman L
Molecular metabolism (2022) 66: 101631. 390 004 IHC; tested species: mouse
Amygdala inhibitory neurons as loci for translation in emotional memories.
Shrestha P, Shan Z, Mamcarz M, Ruiz KSA, Zerihoun AT, Juan CY, Herrero-Vidal PM, Pelletier J, Heintz N, Klann E
Nature (2020) 5867829: 407-411. 390 004 IHC; tested species: mouse
Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome.
Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, et al.
bioRxiv : the preprint server for biology (2023) : . 390 004 IHC-G; tested species: drosophila
Fetal rat neural progenitor cell transplantation after spinal cord injury improves motor recovery following optogenetic stimulation.
Sánchez-Martín MDM, Giraldo E, Alastrue-Agudo A, López-Mocholi E, Martín-Pérez S, Maninno L, Paniagua Soriano G, Fraga Sánchez AI, Monreal-Trigo J, Terrés-Haro JM, Vidal Beneyto Q, et al.
Molecular therapy : the journal of the American Society of Gene Therapy (2025) : . 390 004 ICC, IHC, iDISCO; tested species: rat
WB
ICC
IHC
IHC-P
Background
Red fluorescent protein RFP and its derivates have become universal tools in cell biology. Most RFPs derive from a protein isolated from Discosoma sp. They are used as fluorescent tags to investigate expression levels, patterns and protein localization.
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