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p21-Arc (ARPC3) antibody - 303 011

p21-Arc is a member of the seven protein complex required for actin nucleation
Mouse monoclonal purified IgG
Cat. No.: 303 011
Amount: 100 µg
Price: $420.00
Cat. No. 303 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 500 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 24A6
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 178 from human p21-Arc (UniProt Id: O15145)
Epitop AA 109 to 120 from human p21-Arc (UniProt Id: O15145)
Reactivity Reacts with: human (O15145), rat (Q4V7C7), mouse (Q9JM76), hamster.
Other species not tested yet.
Data sheet 303_011.pdf

References for p21-Arc - 303 011

Aging-Associated Changes in Oxidative Stress Negatively Impacts the Urinary Bladder Urothelium.
de Rijk MM, Wolf-Johnston A, Kullmann AF, Taiclet S, Kanai AJ, Shiva S, Birder LA
International neurourology journal (2022) 262: 111-118. 303 011 WB; tested species: rat
Effects of acute and repeated cocaine on markers for neural plasticity within the mesolimbic system in rats.
Rodriguez-Espinosa N, Fernandez-Espejo E
Psychopharmacology (2015) 2321: 57-62. 303 011 WB
Antiparkinsonian trophic action of glial cell line-derived neurotrophic factor and transforming growth factor β1 is enhanced after co-infusion in rats.
Gonzalez-Aparicio R, Flores JA, Fernandez-Espejo E
Experimental neurology (2010) 2261: 136-47. 303 011 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 303 011 WB
Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes.
Steffen A, Faix J, Resch GP, Linkner J, Wehland J, Small JV, Rottner K, Stradal TE
Molecular biology of the cell (2006) 176: 2581-91. 303 011 WB
Cat. No.: 303 011
Amount: 100 µg
Price: $420.00
Aging-Associated Changes in Oxidative Stress Negatively Impacts the Urinary Bladder Urothelium.
de Rijk MM, Wolf-Johnston A, Kullmann AF, Taiclet S, Kanai AJ, Shiva S, Birder LA
International neurourology journal (2022) 262: 111-118. 303 011 WB; tested species: rat
Effects of acute and repeated cocaine on markers for neural plasticity within the mesolimbic system in rats.
Rodriguez-Espinosa N, Fernandez-Espejo E
Psychopharmacology (2015) 2321: 57-62. 303 011 WB
Antiparkinsonian trophic action of glial cell line-derived neurotrophic factor and transforming growth factor β1 is enhanced after co-infusion in rats.
Gonzalez-Aparicio R, Flores JA, Fernandez-Espejo E
Experimental neurology (2010) 2261: 136-47. 303 011 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 303 011 WB
Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes.
Steffen A, Faix J, Resch GP, Linkner J, Wehland J, Small JV, Rottner K, Stradal TE
Molecular biology of the cell (2006) 176: 2581-91. 303 011 WB
WB
ICC
Background
Actin polymerization is a necessary event for cell motility. Spontanous actin oligomerization is slow at given monomeric actin concentrations in cells. The Arp 2/3 complex which is about 220 kDa in size has turned out to initiate the polymerization of new actin filaments. This complex consists of two actin like proteins Arp2 and Arp3 and five additional proteins: p16-Arc (ArpC5), p20-Arc (ArpC4), p21-Arc (ArpC3), p34-Arc (ArpC2) and p41-Arc (ArpC1). Expression of partial complexes revealed that a heterodimer of p20-Arc and p34-Arc constitutes the core of the complex whereas the remaining subunits are peripherally located.