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alpha-Tubulin antibody - 302 204

alpha-Tubulin is a major cytoskeleton protein
Guinea pig polyclonal antiserum
Cat. No.: 302 204
Amount: 100 µl
Price: $370.00
Cat. No. 302 204 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Reactivity Reacts with: human (P68366), rat (Q5XIF6), mouse (P68368).
Other species not tested yet.
Specificity Specific for α-tubulin (glu- and tyr-α-tubulin).
Matching control protein/peptide 302-21P
Data sheet 302_204.pdf

References for alpha-Tubulin - 302 204

Foxp1 suppresses cortical angiogenesis and attenuates HIF-1alpha signaling to promote neural progenitor cell maintenance.
Buth JE, Dyevich CE, Rubin A, Wang C, Gao L, Marks T, Harrison MR, Kong JH, Ross ME, Novitch BG, Pearson CA, et al.
EMBO reports (2024) : . 302 204 WB; tested species: mouse
Cat. No.: 302 204
Amount: 100 µl
Price: $370.00
Foxp1 suppresses cortical angiogenesis and attenuates HIF-1alpha signaling to promote neural progenitor cell maintenance.
Buth JE, Dyevich CE, Rubin A, Wang C, Gao L, Marks T, Harrison MR, Kong JH, Ross ME, Novitch BG, Pearson CA, et al.
EMBO reports (2024) : . 302 204 WB; tested species: mouse
WB
ICC
IHC