Synaptic Systems

Cat. No.: 262 013

Amount: 50 µg

Price: $380.00

Cat. No. 262 013	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: external data (see remarks) IP: not tested yet ICC: 1 : 200 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 18 to 107 from mouse CAPS1 (UniProt Id: Q80TJ1)
Reactivity	Reacts with: rat (Q62717), mouse (Q80TJ1). Other species not tested yet.
Specificity	Specific for CAPS 1, no cross-reactvity to CAPS 2 K.O. validated PubMed: 25719439
Remarks	WB: Cat. no. 262 003 is recommended for this application. This antibody has not yet been successfully tested for this application using our standard protocol, but has been published by customers (see “application” references).
Data sheet	262_013.pdf

References for CAPS1 - 262 013

Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 262 013 WB; tested species: mouse

Paralogs of the Calcium-Dependent Activator Protein for Secretion Differentially Regulate Synaptic Transmission and Peptide Secretion in Sensory Neurons.
Shaib AH, Staudt A, Harb A, Klose M, Shaaban A, Schirra C, Mohrmann R, Rettig J, Becherer U
Frontiers in cellular neuroscience (2018) 12: 304. 262 013 WB, ICC; KO verified; tested species: mouse

RNA editing-mediated regulation of calcium-dependent activator protein for secretion (CAPS1) localization and its impact on synaptic transmission.
Shumate KM, Tas ST, Kavalali ET, Emeson RB
Journal of neurochemistry (2021) 1582: 182-196. 262 013 ICC; tested species: mouse

Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.
Geerts CJ, Mancini R, Chen N, Koopmans FTW, Li KW, Smit AB, van Weering JRT, Verhage M, Groffen AJA
PloS one (2017) 127: e0180912. 262 013 ICC; tested species: mouse

CAPS-1 promotes fusion competence of stationary dense-core vesicles in presynaptic terminals of mammalian neurons.
Farina M, van de Bospoort R, He E, Persoon CM, van Weering JR, Broeke JH, Verhage M, Toonen RF
eLife (2015) 4: . 262 013 ICC; KO verified

Cat. No.: 262 013

Amount: 50 µg

Price: $380.00

ICC

Background

The Ca²⁺-dependent activator protein for secretion (CAPS) regulates exocytosis of catecholamine- or neuropeptide-containing dense-core vesicles (DCVs) at secretion sites.
Two differen isoforms CAPS 1/CADPS 1 and CAPS 2/CADPS 2 that are mainly expressed in brain have been identified in mammals. Both have been shown to be essential components of the synaptic vesicle priming machinery.

1 ) Sadakata T et al., J. Biol. Chem. (2010)
2 ) Speidel D et al., Cell Metab. (2008)
3 ) Sadakata T et al., J. Histochem. Cytochem. (2007)
4 ) Jockusch WJ et al., Cell (2007)
5 ) Sadakata T et al., J. Comp. Neurol. (2006)
6 ) Speidel D et al., Neuron (2005)

Protocols

ICC

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CAPS1 antibody - 262 013 K.O.

References for CAPS1 - 262 013