Synaptic Systems - GABA-A receptor alpha3

Cat. No.: 224 303

Amount: 50 µg

Price: $460.00

Cat. No. 224 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: external data (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 29 to 43 from rat GABA-A receptor α3 (UniProt Id: P20236)
Reactivity	Reacts with: rat (P20236), mouse (P26049). Other species not tested yet.
Specificity	K.O. validated PubMed: 33119191
Matching control protein/peptide	224-3P
Remarks	IHC: This antibody has been successfully applied for this method by our customers using antigen retrieval with pepsin according to Lorincz and Nusser 2010 (see gallery). It has not been validated using our standard protocol.
Data sheet	224_303.pdf

References for GABA-A receptor alpha3 - 224 303

Deletion of histamine H2 receptor in VTA dopaminergic neurons of mice induces behavior reminiscent of mania.
Ma S, Ma Q, Hu S, Mo X, Zhu C, Zhang X, Jia Z, Tang L, Jiang L, Cui Y, Chen Z, et al.
Cell reports (2024) 439: 114717. 224 303 WB; tested species: mouse

The TMEM132B-GABAA receptor complex controls alcohol actions in the brain.
Wang G, Peng S, Reyes Mendez M, Keramidas A, Castellano D, Wu K, Han W, Tian Q, Dong L, Li Y, Lu W, et al.
Cell (2024) 18723: 6649-6668.e35. 224 303 WB, ICC; tested species: mouse

Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures.
Kreye J, Wright SK, van Casteren A, Stöffler L, Machule ML, Reincke SM, Nikolaus M, van Hoof S, Sanchez-Sendin E, Homeyer MA, Cordero Gómez C, et al.
The Journal of experimental medicine (2021) 21811: . 224 303 ICC; tested species: rat

The α3 subunit of GABAA receptors promotes formation of inhibitory synapses in the absence of collybistin.
Wagner S, Lee C, Rojas L, Specht CG, Rhee J, Brose N, Papadopoulos T
The Journal of biological chemistry () 296: 100709. 224 303 ICC, IHC; tested species: mouse

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 224 303 IHC; tested species: mouse

Cytoplasmic FUS triggers early behavioral alterations linked to cortical neuronal hyperactivity and inhibitory synaptic defects.
Scekic-Zahirovic J, Sanjuan-Ruiz I, Kan V, Megat S, De Rossi P, Dieterlé S, Cassel R, Jamet M, Kessler P, Wiesner D, Tzeplaeff L, et al.
Nature communications (2021) 121: 3028. 224 303 IHC; tested species: mouse

Identification of intraneuronal amyloid beta oligomers in locus coeruleus neurons of Alzheimer's patients and their potential impact on inhibitory neurotransmitter receptors and neuronal excitability.
Kelly L, Seifi M, Ma R, Mitchell SJ, Rudolph U, Viola KL, Klein WL, Lambert JJ, Swinny JD
Neuropathology and applied neurobiology (2021) 474: 488-505. 224 303 IHC; KO verified; tested species: mouse

Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 224 303 IHC; tested species: rat

Spatiotemporal Distribution of GABAA Receptor Subunits Within Layer II of Mouse Medial Entorhinal Cortex: Implications for Grid Cell Excitability.
Berggaard N, Seifi M, van der Want JJL, Swinny JD
Frontiers in neuroanatomy (2018) 12: 46. 224 303 IHC; tested species: mouse

Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala.
Tzanoulinou S, García-Mompó C, Castillo-Gómez E, Veenit V, Nacher J, Sandi C
PloS one (2014) 94: e94666. 224 303 IHC; tested species: rat

Only a Minority of the Inhibitory Inputs to Cerebellar Golgi Cells Originates from Local GABAergic Cells.
Eyre MD, Nusser Z
eNeuro () 32: . 224 303 IHC

Cat. No.: 224 303

Amount: 50 µg

Price: $460.00

Only a Minority of the Inhibitory Inputs to Cerebellar Golgi Cells Originates from Local GABAergic Cells.
Eyre MD, Nusser Z
eNeuro () 32: . 224 303 IHC

ICC

IHC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

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6 ) Viltono L et al., J. Comp. Neurol. (2008)
7 ) Kralic JE et al., J. Comp. Neurol. (2006)
8 ) Essrich C et al., Nat. Neurosci. (1998)
9 ) Fritschy JM et al., J. Comp. Neurol. (1995)
10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

ICC

OTHER

Preabsorbtion

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GABA-A receptor alpha3 antibody extracellular - 224 303 K.O.

References for GABA-A receptor alpha3 - 224 303