Synaptic Systems - Neurobeachin

Cat. No.: 194 003

Amount: 50 µg

Price: $380.00

The STEP61 interactome reveals subunit-specific AMPA receptor binding and synaptic regulation.
Won S, Incontro S, Li Y, Nicoll RA, Roche KW
Proceedings of the National Academy of Sciences of the United States of America (2019) : . 194 003 WB; tested species: mouse

Neurobeachin Regulates Glutamate- and GABA-Receptor Targeting to Synapses via Distinct Pathways.
Farzana F, Zalm R, Chen N, Li KW, Grant SG, Smit AB, Toonen RF, Verhage M
Molecular neurobiology (2016) 534: 2112-23. 194 003 WB, ICC

Neurobeachin regulates neurotransmitter receptor trafficking to synapses.
Nair R, Lauks J, Jung S, Cooke NE, de Wit H, Brose N, Kilimann MW, Verhage M, Rhee J
The Journal of cell biology (2013) 2001: 61-80. 194 003 WB, ICC

Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 194 003 WB, IP, ICC

Dendritic spine formation and synaptic function require neurobeachin.
Niesmann K, Breuer D, Brockhaus J, Born G, Wolff I, Reissner C, Kilimann MW, Rohlmann A, Missler M
Nature communications (2011) 2: 557. 194 003 WB, ICC, IHC

Neurobeachin: A protein kinase A-anchoring, beige/Chediak-higashi protein homolog implicated in neuronal membrane traffic.
Wang X, Herberg FW, Laue MM, Wullner C, Hu B, Petrasch-Parwez E, Kilimann MW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2000) 2023: 8551-65. 194 003 WB, ICC, IHC, EM; tested species: rat

Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 194 003 WB, IP, ICC

Neurobeachin Regulates Glutamate- and GABA-Receptor Targeting to Synapses via Distinct Pathways.
Farzana F, Zalm R, Chen N, Li KW, Grant SG, Smit AB, Toonen RF, Verhage M
Molecular neurobiology (2016) 534: 2112-23. 194 003 WB, ICC

Neurobeachin regulates neurotransmitter receptor trafficking to synapses.
Nair R, Lauks J, Jung S, Cooke NE, de Wit H, Brose N, Kilimann MW, Verhage M, Rhee J
The Journal of cell biology (2013) 2001: 61-80. 194 003 WB, ICC

Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 194 003 WB, IP, ICC

Dendritic spine formation and synaptic function require neurobeachin.
Niesmann K, Breuer D, Brockhaus J, Born G, Wolff I, Reissner C, Kilimann MW, Rohlmann A, Missler M
Nature communications (2011) 2: 557. 194 003 WB, ICC, IHC

Neurobeachin: A protein kinase A-anchoring, beige/Chediak-higashi protein homolog implicated in neuronal membrane traffic.
Wang X, Herberg FW, Laue MM, Wullner C, Hu B, Petrasch-Parwez E, Kilimann MW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2000) 2023: 8551-65. 194 003 WB, ICC, IHC, EM; tested species: rat

Dendritic spine formation and synaptic function require neurobeachin.
Niesmann K, Breuer D, Brockhaus J, Born G, Wolff I, Reissner C, Kilimann MW, Rohlmann A, Missler M
Nature communications (2011) 2: 557. 194 003 WB, ICC, IHC

Neurobeachin: A protein kinase A-anchoring, beige/Chediak-higashi protein homolog implicated in neuronal membrane traffic.
Wang X, Herberg FW, Laue MM, Wullner C, Hu B, Petrasch-Parwez E, Kilimann MW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2000) 2023: 8551-65. 194 003 WB, ICC, IHC, EM; tested species: rat

Protocol for SUM-PAINT spatial proteomic imaging generating neuronal architecture maps in rat hippocampal neurons.
Unterauer EM, Schentarra EM, Jevdokimenko K, Boushehri SS, Marr C, Opazo F, Fornasiero EF, Jungmann R
STAR protocols (2025) 61: 103637. 194 003 DNA-PAINT; tested species: rat

Neurobeachin: A protein kinase A-anchoring, beige/Chediak-higashi protein homolog implicated in neuronal membrane traffic.
Wang X, Herberg FW, Laue MM, Wullner C, Hu B, Petrasch-Parwez E, Kilimann MW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2000) 2023: 8551-65. 194 003 WB, ICC, IHC, EM; tested species: rat

WB

ICC

IHC

Background

Neurobeachin, also referred to as NBEA, is a large ~330 kDa brain-specific protein that belongs to the BEACH (beige and Chediak-Higashi) family. The protein has been shown to locate to tubulovesicular neuronal endomembranes near the trans-Golgi synapses, but also throughout the cell. Neurobeachin binds to protein kinase A and after adding GTPγS concentrates at Golgi-near membranes.

1 ) Su Y et al., J. Neurosci. (2004)
2 ) Wang X et al., J. Neurosci. (2000)

Protocols

WB

IP

Standard protocol

ICC

IHC

Other products for this target

Cat. No. 194 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: not tested yet EM: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to residues near the central region of mouse Neurobeachin (UniProt Id: Q9EPN1)
Reactivity	Reacts with: rat (A0A8I6AB61), mouse (Q9EPN1). Other species not tested yet.
Remarks	WB: To avoid protein aggregation, do not heat samples for SDS-PAGE. Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE. EM: This antibody has been successfully used and published for this application by customers (see “application” references).
Data sheet	194_003.pdf

Neurobeachin antibody - 194 003

References for Neurobeachin - 194 003