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MAP2 antibody - 188 002

MAP2 is a major microtubule associated protein in neurons
Rabbit polyclonal antiserum
Cat. No.: 188 002
Amount: 200 µl
Price: $365.00
Cat. No. 188 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 250 up to 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to residues near the amino terminus of human MAP2 (UniProt Id: P11137-4)
Reactivity Reacts with: human (P11137), rat (P15146), mouse (P20357), chicken.
Other species not tested yet.
Specificity Specific for MAP2; recognizes all four isoforms.
Matching control protein/peptide 188-0P
Remarks

WB: Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE.
IHC: Antigen retrieval with citrate buffer pH 6 is tolerated.
Data sheet 188_002.pdf

References for MAP2 - 188 002

Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development.
Hennegan J, Bryant AH, Griffiths L, Trigano M, Bartley OJM, Bartlett JJ, Minahan C, Abreu de Oliveira WA, Yutuc E, Ntikas S, Bartsocas CS, et al.
iScience (2024) 271: 108670. 188 002 ICC; tested species: human
Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons.
Sousa SC, Aroso M, Bessa R, Veríssimo E, Ferreira da Silva T, Lopes CDF, Brites P, Vieira J, Vieira CP, Aguiar PC, Sousa MM, et al.
Current biology : CB (2024) 3419: 4577-4588.e8. 188 002 ICC; tested species: mouse
Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer's disease tau.
Hromadkova L, Kim C, Haldiman T, Peng L, Zhu X, Cohen M, de Silva R, Safar JG
Cell & bioscience (2023) 131: 174. 188 002 ICC; tested species: mouse
Rebuilding essential active zone functions within a synapse.
Tan C, Wang SSH, de Nola G, Kaeser PS
Neuron (2022) 1109: 1498-1515.e8. 188 002 ICC; tested species: mouse
Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response.
Manet C, Mansuroglu Z, Conquet L, Bortolin V, Comptdaer T, Segrt H, Bourdon M, Menidjel R, Stadler N, Tian G, Herit F, et al.
Journal of neuroinflammation (2022) 191: 307. 188 002 ICC; tested species: mouse
PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure.
Emperador-Melero J, Wong MY, Wang SSH, de Nola G, Nyitrai H, Kirchhausen T, Kaeser PS
Nature communications (2021) 121: 3057. 188 002 ICC; tested species: mouse
IPSC-Derived Neuronal Cultures Carrying the Alzheimer's Disease Associated TREM2 R47H Variant Enables the Construction of an Aβ-Induced Gene Regulatory Network.
Martins S, Müller-Schiffmann A, Erichsen L, Bohndorf M, Wruck W, Sleegers K, Van Broeckhoven C, Korth C, Adjaye J
International journal of molecular sciences (2020) 2112: . 188 002 ICC; tested species: human
Gabapentin inhibits multiple steps in the amyloid beta toxicity cascade.
González-Sanmiguel J, Burgos CF, Bascuñán D, Fernández-Pérez EJ, Riffo-Lepe N, Boopathi S, Fernández-Pérez A, Bobadilla-Azócar C, González W, Figueroa M, Vicente B, et al.
ACS chemical neuroscience (2020) : . 188 002 ICC; tested species: rat
Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ Channels and Ca2+ Entry.
Held RG, Liu C, Ma K, Ramsey AM, Tarr TB, De Nola G, Wang SSH, Wang J, van den Maagdenberg AMJM, Schneider T, Sun J, et al.
Neuron (2020) 1074: 667-683.e9. 188 002 ICC; tested species: mouse
Matrix Metalloproteinase 14 Mediates APP Proteolysis and Lysosomal Alterations Induced by Oxidative Stress in Human Neuronal Cells.
Llorente P, Martins S, Sastre I, Aldudo J, Recuero M, Adjaye J, Bullido MJ
Oxidative medicine and cellular longevity (2020) 2020: 5917187. 188 002 ICC; tested species: human
Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice.
Uezu A, Hisey E, Kobayashi Y, Gao Y, Bradshaw TW, Devlin P, Rodriguiz R, Tata PR, Soderling S
eLife (2019) 8: . 188 002 ICC; tested species: mouse
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 188 002 ICC
The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 188 002 ICC
Modulation of network activity and induction of homeostatic synaptic plasticity by enzymatic removal of heparan sulfates.
Korotchenko S, Cingolani LA, Kuznetsova T, Bologna LL, Chiappalone M, Dityatev A
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2014) 3691654: 20140134. 188 002 ICC; tested species: mouse
Inefficient type I interferon-mediated antiviral protection of primary mouse neurons is associated with the lack of apolipoprotein l9 expression.
Kreit M, Paul S, Knoops L, De Cock A, Sorgeloos F, Michiels T
Journal of virology (2014) 887: 3874-84. 188 002 ICC; tested species: mouse
Somatodendritic serotonin release and re-uptake in mouse embryonic stem cell-derived serotonergic neurons.
Lau T, Schneidt T, Heimann F, Gundelfinger ED, Schloss P
Neurochemistry international (2010) 578: 969-78. 188 002 ICC
Contextual fear response is modulated by M-type K+ channels and is associated with subtle structural changes of the axon initial segment in hippocampal GABAergic neurons.
Ruiz SA, Tikochinsky E, Rubovitch V, Pick CG, Attali B
AIMS neuroscience (2023) 101: 33-51. 188 002 IHC; tested species: mouse
AAV-Mediated CRISPRi and RNAi Based Gene Silencing in Mouse Hippocampal Neurons.
Deutsch M, Günther A, Lerchundi R, Rose CR, Balfanz S, Baumann A
Cells (2021) 102: . 188 002 IHC; tested species: mouse
Role of endocannabinoid signaling in a septohabenular pathway in the regulation of anxiety- and depressive-like behavior.
Vickstrom CR, Liu X, Liu S, Hu MM, Mu L, Hu Y, Yu H, Love SL, Hillard CJ, Liu QS
Molecular psychiatry (2020) : . 188 002 IHC; tested species: mouse
Up-regulation of neurofilament light chains is associated with diminished immunoreactivities for MAP2 and tau after ischemic stroke in rodents and in a human case.
Härtig W, Krueger M, Hofmann S, Preißler H, Märkel M, Frydrychowicz C, Mueller WC, Bechmann I, Michalski D
Journal of chemical neuroanatomy (2016) 78: 140-148. 188 002 IHC
Region-specific expression of vesicular glutamate and GABA transporters under various ischaemic conditions in mouse forebrain and retina.
Michalski D, Härtig W, Krügel K, Edwards RH, Böddener M, Böhme L, Pannicke T, Reichenbach A, Grosche A
Neuroscience (2013) 231: 328-44. 188 002 IHC
Neurological impairment in experimental antiphospholipid syndrome is associated with increased ligand binding to hippocampal and cortical serotonergic 5-HT1A receptors.
Frauenknecht K, Katzav A, Grimm C, Chapman J, Sommer CJ
Immunobiology (2013) 2184: 517-26. 188 002 IHC; tested species: mouse
Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 188 002 IHC
The anaphase promoting complex is required for memory function in mice.
Kuczera T, Stilling RM, Hsia HE, Bahari-Javan S, Irniger S, Nasmyth K, Sananbenesi F, Fischer A
Learning & memory (Cold Spring Harbor, N.Y.) (2011) 181: 49-57. 188 002 IHC
CD8+ T-Lymphocyte-Driven Limbic Encephalitis Results in Temporal Lobe Epilepsy.
Pitsch J, van Loo KMJ, Gallus M, Dik A, Kamalizade D, Baumgart AK, Gnatkovsky V, Müller JA, Opitz T, Hicking G, Naik VN, et al.
Annals of neurology (2021) 894: 666-685. 188 002 IHC-P; tested species: mouse
Cat. No.: 188 002
Amount: 200 µl
Price: $365.00
Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development.
Hennegan J, Bryant AH, Griffiths L, Trigano M, Bartley OJM, Bartlett JJ, Minahan C, Abreu de Oliveira WA, Yutuc E, Ntikas S, Bartsocas CS, et al.
iScience (2024) 271: 108670. 188 002 ICC; tested species: human
Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons.
Sousa SC, Aroso M, Bessa R, Veríssimo E, Ferreira da Silva T, Lopes CDF, Brites P, Vieira J, Vieira CP, Aguiar PC, Sousa MM, et al.
Current biology : CB (2024) 3419: 4577-4588.e8. 188 002 ICC; tested species: mouse
Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer's disease tau.
Hromadkova L, Kim C, Haldiman T, Peng L, Zhu X, Cohen M, de Silva R, Safar JG
Cell & bioscience (2023) 131: 174. 188 002 ICC; tested species: mouse
Rebuilding essential active zone functions within a synapse.
Tan C, Wang SSH, de Nola G, Kaeser PS
Neuron (2022) 1109: 1498-1515.e8. 188 002 ICC; tested species: mouse
Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response.
Manet C, Mansuroglu Z, Conquet L, Bortolin V, Comptdaer T, Segrt H, Bourdon M, Menidjel R, Stadler N, Tian G, Herit F, et al.
Journal of neuroinflammation (2022) 191: 307. 188 002 ICC; tested species: mouse
PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure.
Emperador-Melero J, Wong MY, Wang SSH, de Nola G, Nyitrai H, Kirchhausen T, Kaeser PS
Nature communications (2021) 121: 3057. 188 002 ICC; tested species: mouse
IPSC-Derived Neuronal Cultures Carrying the Alzheimer's Disease Associated TREM2 R47H Variant Enables the Construction of an Aβ-Induced Gene Regulatory Network.
Martins S, Müller-Schiffmann A, Erichsen L, Bohndorf M, Wruck W, Sleegers K, Van Broeckhoven C, Korth C, Adjaye J
International journal of molecular sciences (2020) 2112: . 188 002 ICC; tested species: human
Gabapentin inhibits multiple steps in the amyloid beta toxicity cascade.
González-Sanmiguel J, Burgos CF, Bascuñán D, Fernández-Pérez EJ, Riffo-Lepe N, Boopathi S, Fernández-Pérez A, Bobadilla-Azócar C, González W, Figueroa M, Vicente B, et al.
ACS chemical neuroscience (2020) : . 188 002 ICC; tested species: rat
Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ Channels and Ca2+ Entry.
Held RG, Liu C, Ma K, Ramsey AM, Tarr TB, De Nola G, Wang SSH, Wang J, van den Maagdenberg AMJM, Schneider T, Sun J, et al.
Neuron (2020) 1074: 667-683.e9. 188 002 ICC; tested species: mouse
Matrix Metalloproteinase 14 Mediates APP Proteolysis and Lysosomal Alterations Induced by Oxidative Stress in Human Neuronal Cells.
Llorente P, Martins S, Sastre I, Aldudo J, Recuero M, Adjaye J, Bullido MJ
Oxidative medicine and cellular longevity (2020) 2020: 5917187. 188 002 ICC; tested species: human
Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice.
Uezu A, Hisey E, Kobayashi Y, Gao Y, Bradshaw TW, Devlin P, Rodriguiz R, Tata PR, Soderling S
eLife (2019) 8: . 188 002 ICC; tested species: mouse
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 188 002 ICC
The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 188 002 ICC
Modulation of network activity and induction of homeostatic synaptic plasticity by enzymatic removal of heparan sulfates.
Korotchenko S, Cingolani LA, Kuznetsova T, Bologna LL, Chiappalone M, Dityatev A
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2014) 3691654: 20140134. 188 002 ICC; tested species: mouse
Inefficient type I interferon-mediated antiviral protection of primary mouse neurons is associated with the lack of apolipoprotein l9 expression.
Kreit M, Paul S, Knoops L, De Cock A, Sorgeloos F, Michiels T
Journal of virology (2014) 887: 3874-84. 188 002 ICC; tested species: mouse
Somatodendritic serotonin release and re-uptake in mouse embryonic stem cell-derived serotonergic neurons.
Lau T, Schneidt T, Heimann F, Gundelfinger ED, Schloss P
Neurochemistry international (2010) 578: 969-78. 188 002 ICC
Contextual fear response is modulated by M-type K+ channels and is associated with subtle structural changes of the axon initial segment in hippocampal GABAergic neurons.
Ruiz SA, Tikochinsky E, Rubovitch V, Pick CG, Attali B
AIMS neuroscience (2023) 101: 33-51. 188 002 IHC; tested species: mouse
AAV-Mediated CRISPRi and RNAi Based Gene Silencing in Mouse Hippocampal Neurons.
Deutsch M, Günther A, Lerchundi R, Rose CR, Balfanz S, Baumann A
Cells (2021) 102: . 188 002 IHC; tested species: mouse
Role of endocannabinoid signaling in a septohabenular pathway in the regulation of anxiety- and depressive-like behavior.
Vickstrom CR, Liu X, Liu S, Hu MM, Mu L, Hu Y, Yu H, Love SL, Hillard CJ, Liu QS
Molecular psychiatry (2020) : . 188 002 IHC; tested species: mouse
Up-regulation of neurofilament light chains is associated with diminished immunoreactivities for MAP2 and tau after ischemic stroke in rodents and in a human case.
Härtig W, Krueger M, Hofmann S, Preißler H, Märkel M, Frydrychowicz C, Mueller WC, Bechmann I, Michalski D
Journal of chemical neuroanatomy (2016) 78: 140-148. 188 002 IHC
Region-specific expression of vesicular glutamate and GABA transporters under various ischaemic conditions in mouse forebrain and retina.
Michalski D, Härtig W, Krügel K, Edwards RH, Böddener M, Böhme L, Pannicke T, Reichenbach A, Grosche A
Neuroscience (2013) 231: 328-44. 188 002 IHC
Neurological impairment in experimental antiphospholipid syndrome is associated with increased ligand binding to hippocampal and cortical serotonergic 5-HT1A receptors.
Frauenknecht K, Katzav A, Grimm C, Chapman J, Sommer CJ
Immunobiology (2013) 2184: 517-26. 188 002 IHC; tested species: mouse
Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 188 002 IHC
The anaphase promoting complex is required for memory function in mice.
Kuczera T, Stilling RM, Hsia HE, Bahari-Javan S, Irniger S, Nasmyth K, Sananbenesi F, Fischer A
Learning & memory (Cold Spring Harbor, N.Y.) (2011) 181: 49-57. 188 002 IHC
CD8+ T-Lymphocyte-Driven Limbic Encephalitis Results in Temporal Lobe Epilepsy.
Pitsch J, van Loo KMJ, Gallus M, Dik A, Kamalizade D, Baumgart AK, Gnatkovsky V, Müller JA, Opitz T, Hicking G, Naik VN, et al.
Annals of neurology (2021) 894: 666-685. 188 002 IHC-P; tested species: mouse
WB
ICC
ICC
ICC
IHC
IHC
IHC
IHC
IHC
IHC-P
Background

There are two major classes of heat-stable microtubule-associated proteins (MAPs): MAP2 and tau (MAPT). Both bind microtubules and regulate their polymerization and stability—a critical process for maintaining cellular architecture and dynamics (1).


MAP2 exists in four main isoforms—MAP2A, MAP2B, MAP2C, and MAP2D—via alternative splicing. The high molecular weight isoforms MAP2A/B (~250 kDa) and lower molecular weight isoforms MAP2C/D (~70 kDa) all share a conserved microtubule-binding core domain, important for dendritic stabilization and neuritogenesis (2).


Since microtubule dynamics are central to cell division, migration, and morphology, aberrations in MAP2 and tau expression have been implicated in several types of cancer. 


Consequently, MAP2 expression has diagnostic and prognostic relevance in neuro-oncology. MAP2 immunoreactivity helps distinguish glial neoplasms in neuropathology, and its expression tends to vary according to tumor grade (3). While classic low-grade gliomas often show robust MAP2 staining, higher-grade tumors may exhibit less-specific and more heterogeneous patterns. Moreover, in melanoma, reduced MAP2 expression correlates with increased tumor aggressiveness, underscoring its potential role as a tumor suppressive marker (4).
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