Vimentin antibody - 172 004

Vimentin is a type III intermediate filament

Guinea pig polyclonal antiserum

Cat. No.: 172 004

Amount: 100 µl

Price: $370.00

Cat. No. 172 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 250 up to 1 : 1000 gallery ExM: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Recombinant protein corresponding to AA 1 to 466 from mouse Vimentin (UniProt Id: P20152)
Reactivity	Reacts with: mouse (P20152), rat (P31000). Other species not tested yet.
Remarks	ExM: This antibody has been successfully used in LICONN expansion microscopy method (Tavakoli et al. 2025, PMID: 40335689).
Data sheet	172_004.pdf

References for Vimentin - 172 004

Light-microscopy-based connectomic reconstruction of mammalian brain tissue.
Tavakoli MR, Lyudchik J, Januszewski M, Vistunou V, Agudelo Dueñas N, Vorlaufer J, Sommer C, Kreuzinger C, Oliveira B, Cenameri A, Novarino G, et al.
Nature (2025) 6428067: 398-410. 172 004 ExM; tested species: mouse

Cat. No.: 172 004

Amount: 100 µl

Price: $370.00

ICC

IHC

IHC-P

Background

Vimentin belongs to the family of intermediate filaments that can be subdivided into six major groups based on sequence similarity. Vimentin belongs to the type III category and is the predominant subunit protein of intermediate filaments in tissues of mesenchymal origin.
Like other intermediate filaments it plays a role in the cytoskeletal organization and maintenance of cell shape and morphology.

1 ) Styers ML et al., J. Cell. Sci. (2006)
2 ) Esue O et al., J. Biol. Chem. (2006)
3 ) Miyaguchi K et al., J. Struct. Biol. (1997)
4 ) Chiu FC et al., J. Neurochem. (1981)
5 ) Browning ET et al., J. Cell Biol. (1981)
6 ) Gard DL et al., Cell (1980)

Protocols

ICC

IHC

IHC-P

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