Cat. No.: 160 111AbOR
Amount: 100 µg
Price:
$470.00
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| Cat. No. 160 111AbOR |
100 µg purified IgG, lyophilized, fluorescence-labeled with
abberior STAR ORANGE.
Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications | |
| Label | abberior STAR ORANGE |
| Clone | SY-72G2 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Recombinant protein corresponding to the c-terminal half of human Homer1b (UniProt Id: Q86YM7-1) |
| Reactivity |
Reacts with: mouse (Q9Z2Y3), rat (Q9Z214), human (Q86YM7-1). Other species not tested yet. |
| Specificity | Specific for Homer1b and 1c; no cross-reactivity to Homer1a |
| Remarks |
ICC: This antibody conjugate is especially suitable for high resolution STED microscopy. |
| Data sheet | 160_111abor.pdf |
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Homer is a scaffolding protein localized in the postsynaptic density (PSD) and is highly enriched at excitatory synapses. It acts as a molecular adaptor by binding to metabotropic glutamate receptors (mGluRs) (1), TRPC1 channels, Shank family proteins (2), and several other signaling molecules, organizing them into distinct clusters and thereby establishing specific signaling domains within the PSD.
By cross-linking these proteins, Homer plays a crucial role in structural and functional organization of the PSD, contributing to the maturation of dendritic spines and the regulation of synaptic plasticity. Homer and Shank, in particular, form a mesh-like matrix that serves as a platform for assembly of other PSD proteins (3).
There are three main Homer isoforms—Homer1, Homer2, and Homer3—each of which is subject to alternative splicing, producing multiple splice variants such as a, b, c, and d. These variants can have distinct functional properties, and their dynamic redistribution at synapses is involved in remodeling the PSD in response to neuronal activity (4).
Emerging evidence suggests broader roles for Homer1b/c beyond synaptic scaffolding, including in non-neuronal contexts, although their specific involvement in cancer remains unclear (5).