Cat. No.: 160-02P
Amount: 100 µg
Price:
$110.00
|
|
|
| Cat. No. 160-02P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
|
| Immunogen | Recombinant protein corresponding to the c-terminal half of human Homer 1b (UniProt Id: Q86YM7-1) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 160 022, 160 023 |
| Remarks |
This control protein consists of the recombinant protein (aa 152-354 of human homer 1b) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 160-02p.pdf |
|
|
Homer is a scaffolding protein localized in the postsynaptic density (PSD) and is highly enriched at excitatory synapses. It acts as a molecular adaptor by binding to metabotropic glutamate receptors (mGluRs) (1), TRPC1 channels, Shank family proteins (2), and several other signaling molecules, organizing them into distinct clusters and thereby establishing specific signaling domains within the PSD.
By cross-linking these proteins, Homer plays a crucial role in structural and functional organization of the PSD, contributing to the maturation of dendritic spines and the regulation of synaptic plasticity. Homer and Shank, in particular, form a mesh-like matrix that serves as a platform for assembly of other PSD proteins (3).
There are three main Homer isoforms—Homer1, Homer2, and Homer3—each of which is subject to alternative splicing, producing multiple splice variants such as a, b, c, and d. These variants can have distinct functional properties, and their dynamic redistribution at synapses is involved in remodeling the PSD in response to neuronal activity (4).
Emerging evidence suggests broader roles for Homer1b/c beyond synaptic scaffolding, including in non-neuronal contexts, although their specific involvement in cancer remains unclear (5).