Cat. No.: 140 205
Amount: 50 µg
Price:
$465.00
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| Cat. No. 140 205 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to AA 1 to 466 from rat Rim2 (UniProt Id: Q9JIS1) |
| Reactivity |
Reacts with: rat (Q9JIS1), mouse (Q9EQZ7). Other species not tested yet. |
| Specificity | RIM 2 including splice variants, cross reacts to RIM 1. |
| Remarks |
IHC: This antibody has not yet been successfully tested for this application using our standard protocol, but has been published by customers (see IHC references). This antibody has been successfully used in LICONN expansion microscopy method (Tavakoli et al. 2025, PMID: 40335689). |
| Data sheet | 140_205.pdf |
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RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.
RIM 2β consists of a specific N-terminus, the central PDZ domain and the C-terminal C2 domains. The mRNA for RIM 2β is transcribed from an internal promoter of the RIM 2α gene.
Shorter variants of RIM 2 which comprise only the C-terminal C2B domain and some flanking regions are referred to as NIM 2 / RIM 2γ and NIM 3 / RIM 3γ.