Cat. No.: 140 105
Amount: 50 µg
Price:
$465.00
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| Cat. No. 140 105 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to AA 461 to 987 from rat Rim2 (UniProt Id: Q9JIS1) |
| Reactivity |
Reacts with: rat (Q9JIS1), mouse (Q9EQZ7). Other species not tested yet. |
| Specificity | RIM 2 including splice variants, weak cross reactivity to RIM 1. |
| Remarks |
IHC: This antibody has been successfully applied for this method by our customers using antigen retrieval with pepsin according to Lorincz and Nusser 2010 (see gallery). It has not been validated using our standard protocol. |
| Data sheet | 140_105.pdf |
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RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 2β consists of a specific N-terminus, the central PDZ domain and the C-terminal C2 domains. The mRNA for RIM 2β is transcribed from an internal promoter of the RIM 2α gene.
Shorter variants of RIM 2 which comprise only the C-terminal C2B domain and some flanking regions are referred to as NIM 2 / RIM 2γ and NIM 3 / RIM 3γ.