RIM1 antibody PDZ domain - 140 005

RIMs are Rab3 effector proteins of the cytomatrix at the active zone

Guinea pig polyclonal purified antibody

Cat. No.: 140 005

Amount: 50 µg

Price: $465.00

Cat. No. 140 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: external data (see remarks) gallery IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 596 to 705 from rat Rim1 (UniProt Id: Q9JIR4)
Reactivity	Reacts with: rat (Q9JIR4), mouse (Q99NE5). Other species not tested yet.
Specificity	Specific for RIM 1 with weak cross reactivity to RIM 2.
Remarks	IHC: This antibody has been successfully applied for this method by our customers using antigen retrieval with pepsin according to Lorincz and Nusser 2010 (see gallery). It has not been validated using our standard protocol.
Data sheet	140_005.pdf

References for RIM1 - 140 005

Correction of amygdalar dysfunction in a rat model of fragile X syndrome.
Fernandes G, Mishra PK, Nawaz MS, Donlin-Asp PG, Rahman MM, Hazra A, Kedia S, Kayenaat A, Songara D, Wyllie DJA, Schuman EM, et al.
Cell reports (2021) 372: 109805. 140 005 IHC; tested species: rat

Cat. No.: 140 005

Amount: 50 µg

Price: $465.00

ICC

IHC

Background

RIMs are presynaptic active zone proteins that regulate Ca²⁺ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.

1 ) Wang Y et al., Genomics (2003)
2 ) Castillo PE et al., Nature (2002)
3 ) Schoch S et al., Nature (2002)
4 ) Wang Y et al., J. Biol. Chem. (2000)
5 ) Wang Y et al., Nature (1997)

Protocols

ICC

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