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alpha Synuclein antibody - 128 211

Synucleins are involved in Parkinson's and Alzheimer's Disease
Mouse monoclonal purified IgG
Cat. No.: 128 211
Amount: 100 µg
Price: $425.00
Cat. No. 128 211 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
DNA-PAINT: external data (see remarks)
ELISA: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone 354A10
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of human α-Synuclein. (UniProt Id: P37840)
Reactivity Reacts with: human (P37840), rat (P37377), mouse (O55042), mammals.
Other species not tested yet.
Specificity Specific for α-synuclein, no cross-reactivity to β- and γ-synuclein.
Remarks

DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
ELISA: Suitable as capture antibody for sandwich-ELISA. Please refer to the protocol for suitable detector antibodies.
Data sheet 128_211.pdf

References for alpha Synuclein - 128 211

Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons.
Parra-Rivas LA, Sharma R, Rust TE, Bazick HO, Carlson-Stevermer J, Zylka MJ, Ogawa Y, Roy S
STAR protocols (2025) 63: 103945. 128 211 WB, ICC; tested species: human
Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function.
Parra-Rivas LA, Madhivanan K, Aulston BD, Wang L, Prakashchand DD, Boyer NP, Saia-Cereda VM, Branes-Guerrero K, Pizzo DP, Bagchi P, Sundar VS, et al.
Neuron (2023) 11124: 4006-4023.e10. 128 211 WB, IHC; tested species: mouse
N-terminal acetylation mutants affect alpha-synuclein stability, protein levels and neuronal toxicity.
Vinueza-Gavilanes R, Íñigo-Marco I, Larrea L, Lasa M, Carte B, Santamaría E, Fernández-Irigoyen J, Bugallo R, Aragón T, Aldabe R, Arrasate M, et al.
Neurobiology of disease (2020) : 104781. 128 211 WB, ICC; tested species: human
Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons.
Parra-Rivas LA, Sharma R, Rust TE, Bazick HO, Carlson-Stevermer J, Zylka MJ, Ogawa Y, Roy S
STAR protocols (2025) 63: 103945. 128 211 WB, ICC; tested species: human
Stabilization of 14-3-3 protein-protein interactions with Fusicoccin-A decreases alpha-synuclein dependent cell-autonomous death in neuronal and mouse models.
Vinueza-Gavilanes R, Bravo-González JJ, Basurco L, Boncristiani C, Fernández-Irigoyen J, Santamaría E, Marcilla I, Pérez-Mediavilla A, Luquin MR, Vales A, González-Aseguinolaza G, et al.
Neurobiology of disease (2023) : 106166. 128 211 ICC; tested species: mouse
N-terminal acetylation mutants affect alpha-synuclein stability, protein levels and neuronal toxicity.
Vinueza-Gavilanes R, Íñigo-Marco I, Larrea L, Lasa M, Carte B, Santamaría E, Fernández-Irigoyen J, Bugallo R, Aragón T, Aldabe R, Arrasate M, et al.
Neurobiology of disease (2020) : 104781. 128 211 WB, ICC; tested species: human
E46K α-synuclein pathological mutation causes cell-autonomous toxicity without altering protein turnover or aggregation.
Íñigo-Marco I, Valencia M, Larrea L, Bugallo R, Martínez-Goikoetxea M, Zuriguel I, Arrasate M
Proceedings of the National Academy of Sciences of the United States of America (2017) 11439: E8274-E8283. 128 211 ICC; tested species: rat
Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function.
Parra-Rivas LA, Madhivanan K, Aulston BD, Wang L, Prakashchand DD, Boyer NP, Saia-Cereda VM, Branes-Guerrero K, Pizzo DP, Bagchi P, Sundar VS, et al.
Neuron (2023) 11124: 4006-4023.e10. 128 211 WB, IHC; tested species: mouse
One-step nanoscale expansion microscopy reveals individual protein shapes.
Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, et al.
Nature biotechnology (2024) : . 128 211 ExM; tested species: rat
Protocol for SUM-PAINT spatial proteomic imaging generating neuronal architecture maps in rat hippocampal neurons.
Unterauer EM, Schentarra EM, Jevdokimenko K, Boushehri SS, Marr C, Opazo F, Fornasiero EF, Jungmann R
STAR protocols (2025) 61: 103637. 128 211 DNA-PAINT; tested species: rat
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 128 211 DNA-PAINT; tested species: rat
Multiplex imaging of human induced pluripotent stem cell-derived neurons with CO-Detection by indEXing (CODEX) technology.
Heinrich L, Zafar F, Morato Torres CA, Singh J, Khan A, Chen MY, Hempel C, Nikulina N, Mulholland J, Braubach O, Schüle B, et al.
Journal of neuroscience methods (2022) : 109653. 128 211 Codex-PC; tested species: human
Cat. No.: 128 211
Amount: 100 µg
Price: $425.00
Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons.
Parra-Rivas LA, Sharma R, Rust TE, Bazick HO, Carlson-Stevermer J, Zylka MJ, Ogawa Y, Roy S
STAR protocols (2025) 63: 103945. 128 211 WB, ICC; tested species: human
Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function.
Parra-Rivas LA, Madhivanan K, Aulston BD, Wang L, Prakashchand DD, Boyer NP, Saia-Cereda VM, Branes-Guerrero K, Pizzo DP, Bagchi P, Sundar VS, et al.
Neuron (2023) 11124: 4006-4023.e10. 128 211 WB, IHC; tested species: mouse
N-terminal acetylation mutants affect alpha-synuclein stability, protein levels and neuronal toxicity.
Vinueza-Gavilanes R, Íñigo-Marco I, Larrea L, Lasa M, Carte B, Santamaría E, Fernández-Irigoyen J, Bugallo R, Aragón T, Aldabe R, Arrasate M, et al.
Neurobiology of disease (2020) : 104781. 128 211 WB, ICC; tested species: human
Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons.
Parra-Rivas LA, Sharma R, Rust TE, Bazick HO, Carlson-Stevermer J, Zylka MJ, Ogawa Y, Roy S
STAR protocols (2025) 63: 103945. 128 211 WB, ICC; tested species: human
Stabilization of 14-3-3 protein-protein interactions with Fusicoccin-A decreases alpha-synuclein dependent cell-autonomous death in neuronal and mouse models.
Vinueza-Gavilanes R, Bravo-González JJ, Basurco L, Boncristiani C, Fernández-Irigoyen J, Santamaría E, Marcilla I, Pérez-Mediavilla A, Luquin MR, Vales A, González-Aseguinolaza G, et al.
Neurobiology of disease (2023) : 106166. 128 211 ICC; tested species: mouse
N-terminal acetylation mutants affect alpha-synuclein stability, protein levels and neuronal toxicity.
Vinueza-Gavilanes R, Íñigo-Marco I, Larrea L, Lasa M, Carte B, Santamaría E, Fernández-Irigoyen J, Bugallo R, Aragón T, Aldabe R, Arrasate M, et al.
Neurobiology of disease (2020) : 104781. 128 211 WB, ICC; tested species: human
E46K α-synuclein pathological mutation causes cell-autonomous toxicity without altering protein turnover or aggregation.
Íñigo-Marco I, Valencia M, Larrea L, Bugallo R, Martínez-Goikoetxea M, Zuriguel I, Arrasate M
Proceedings of the National Academy of Sciences of the United States of America (2017) 11439: E8274-E8283. 128 211 ICC; tested species: rat
Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function.
Parra-Rivas LA, Madhivanan K, Aulston BD, Wang L, Prakashchand DD, Boyer NP, Saia-Cereda VM, Branes-Guerrero K, Pizzo DP, Bagchi P, Sundar VS, et al.
Neuron (2023) 11124: 4006-4023.e10. 128 211 WB, IHC; tested species: mouse
One-step nanoscale expansion microscopy reveals individual protein shapes.
Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, et al.
Nature biotechnology (2024) : . 128 211 ExM; tested species: rat
Protocol for SUM-PAINT spatial proteomic imaging generating neuronal architecture maps in rat hippocampal neurons.
Unterauer EM, Schentarra EM, Jevdokimenko K, Boushehri SS, Marr C, Opazo F, Fornasiero EF, Jungmann R
STAR protocols (2025) 61: 103637. 128 211 DNA-PAINT; tested species: rat
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 128 211 DNA-PAINT; tested species: rat
Multiplex imaging of human induced pluripotent stem cell-derived neurons with CO-Detection by indEXing (CODEX) technology.
Heinrich L, Zafar F, Morato Torres CA, Singh J, Khan A, Chen MY, Hempel C, Nikulina N, Mulholland J, Braubach O, Schüle B, et al.
Journal of neuroscience methods (2022) : 109653. 128 211 Codex-PC; tested species: human
WB
ICC
ICC
IHC
IHC-P
Background
Synuclein proteins are produced by three genes. They share structural resemblance to apolipoproteins, but are abundant in the neuronal cytosol and present in enriched amounts at presynaptic terminals.
Synucleins have been specifically implicated in three diseases: Alzheimer's (AD), Parkinson's (PD) and breast cancer. In AD, a peptide derived from α-synuclein forms an intrinsic component of plaque amyloid. In PD, an α-synuclein allele is genetically linked to several independent familial cases, and the protein appears to accumulate in Lewy bodies. In breast cancer, increased expression of γ-synuclein correlates with disease progression.
In songbirds, α-synuclein expression is correlated with plasticity in the developing song control system. Although the normal function of synucleins is unknown, a role in synaptic plasticity seems likely.
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