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SNAP47 antibody - 111 403 K.D.

SNAP47 is a novel member of the Qbc SNARE family
Rabbit polyclonal purified antibody
Cat. No.: 111 403
Amount: 50 µg
Price: $380.00
Cat. No. 111 403 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: external data (see remarks) gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 419 from rat SNAP47 (UniProt Id: Q6P6S0)
Reactivity Reacts with: rat (Q6P6S0), mouse (Q8R570).
No signal: zebrafish.
Other species not tested yet.
Specificity K.D. validated PubMed: 33567284
Remarks

IHC: This antibody has been successfully applied for this method by our customers using mild fixation (4% PFA and 15% picric acid) according to Kirizs et al. 2014 (see gallery). It has not been validated using our standard protocol.
Data sheet 111_403.pdf

References for SNAP47 - 111 403

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 111 403 WB; tested species: mouse
TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47.
Urbina FL, Menon S, Goldfarb D, Edwards R, Ben Major M, Brennwald P, Gupton SL
Cell reports (2021) 346: 108743. 111 403 WB, IP, ICC; KD verified; tested species: mouse
SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.
Cardenas RA, Gonzalez R, Sanchez E, Ramos MA, Cardenas EI, Rodarte AI, Alcazar-Felix RJ, Isaza A, Burns AR, Heidelberger R, Adachi R, et al.
The Journal of biological chemistry (2021) 296: 100268. 111 403 WB; tested species: mouse
SNAP-25 isoforms differentially regulate synaptic transmission and long-term synaptic plasticity at central synapses.
Irfan M, Gopaul KR, Miry O, Hökfelt T, Stanton PK, Bark C
Scientific reports (2019) 91: 6403. 111 403 WB; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 111 403 WB; tested species: mouse
SNAP-25 gene family members differentially support secretory vesicle fusion.
Arora S, Saarloos I, Kooistra R, van de Bospoort R, Verhage M, Toonen RF
Journal of cell science (2017) 13011: 1877-1889. 111 403 WB, ICC
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 111 403 WB; tested species: mouse
The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 111 403 WB; tested species: mouse
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 111 403 WB; tested species: mouse
Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression.
Holt M, Varoqueaux F, Wiederhold K, Takamori S, Urlaub H, Fasshauer D, Jahn R
The Journal of biological chemistry (2006) 28125: 17076-83. 111 403 WB, ICC
TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47.
Urbina FL, Menon S, Goldfarb D, Edwards R, Ben Major M, Brennwald P, Gupton SL
Cell reports (2021) 346: 108743. 111 403 WB, IP, ICC; KD verified; tested species: mouse
TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47.
Urbina FL, Menon S, Goldfarb D, Edwards R, Ben Major M, Brennwald P, Gupton SL
Cell reports (2021) 346: 108743. 111 403 WB, IP, ICC; KD verified; tested species: mouse
SNAP-25 gene family members differentially support secretory vesicle fusion.
Arora S, Saarloos I, Kooistra R, van de Bospoort R, Verhage M, Toonen RF
Journal of cell science (2017) 13011: 1877-1889. 111 403 WB, ICC
Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression.
Holt M, Varoqueaux F, Wiederhold K, Takamori S, Urlaub H, Fasshauer D, Jahn R
The Journal of biological chemistry (2006) 28125: 17076-83. 111 403 WB, ICC
Neuronal SNAP-23 is critical for synaptic plasticity and spatial memory independently of NMDA receptor regulation.
Huang M, Bin NR, Rai J, Ma K, Chow CH, Eide S, Harada H, Xiao J, Feng D, Sun HS, Feng ZP, et al.
iScience (2023) 265: 106664. 111 403 IHC; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 111 403 DNA-PAINT; tested species: rat
Cat. No.: 111 403
Amount: 50 µg
Price: $380.00
Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 111 403 WB; tested species: mouse
TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47.
Urbina FL, Menon S, Goldfarb D, Edwards R, Ben Major M, Brennwald P, Gupton SL
Cell reports (2021) 346: 108743. 111 403 WB, IP, ICC; KD verified; tested species: mouse
SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.
Cardenas RA, Gonzalez R, Sanchez E, Ramos MA, Cardenas EI, Rodarte AI, Alcazar-Felix RJ, Isaza A, Burns AR, Heidelberger R, Adachi R, et al.
The Journal of biological chemistry (2021) 296: 100268. 111 403 WB; tested species: mouse
SNAP-25 isoforms differentially regulate synaptic transmission and long-term synaptic plasticity at central synapses.
Irfan M, Gopaul KR, Miry O, Hökfelt T, Stanton PK, Bark C
Scientific reports (2019) 91: 6403. 111 403 WB; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 111 403 WB; tested species: mouse
SNAP-25 gene family members differentially support secretory vesicle fusion.
Arora S, Saarloos I, Kooistra R, van de Bospoort R, Verhage M, Toonen RF
Journal of cell science (2017) 13011: 1877-1889. 111 403 WB, ICC
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 111 403 WB; tested species: mouse
The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 111 403 WB; tested species: mouse
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 111 403 WB; tested species: mouse
Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression.
Holt M, Varoqueaux F, Wiederhold K, Takamori S, Urlaub H, Fasshauer D, Jahn R
The Journal of biological chemistry (2006) 28125: 17076-83. 111 403 WB, ICC
TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47.
Urbina FL, Menon S, Goldfarb D, Edwards R, Ben Major M, Brennwald P, Gupton SL
Cell reports (2021) 346: 108743. 111 403 WB, IP, ICC; KD verified; tested species: mouse
TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47.
Urbina FL, Menon S, Goldfarb D, Edwards R, Ben Major M, Brennwald P, Gupton SL
Cell reports (2021) 346: 108743. 111 403 WB, IP, ICC; KD verified; tested species: mouse
SNAP-25 gene family members differentially support secretory vesicle fusion.
Arora S, Saarloos I, Kooistra R, van de Bospoort R, Verhage M, Toonen RF
Journal of cell science (2017) 13011: 1877-1889. 111 403 WB, ICC
Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression.
Holt M, Varoqueaux F, Wiederhold K, Takamori S, Urlaub H, Fasshauer D, Jahn R
The Journal of biological chemistry (2006) 28125: 17076-83. 111 403 WB, ICC
Neuronal SNAP-23 is critical for synaptic plasticity and spatial memory independently of NMDA receptor regulation.
Huang M, Bin NR, Rai J, Ma K, Chow CH, Eide S, Harada H, Xiao J, Feng D, Sun HS, Feng ZP, et al.
iScience (2023) 265: 106664. 111 403 IHC; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 111 403 DNA-PAINT; tested species: rat
WB
ICC
IHC
Background

The membrane bound protein SNAP47 is abundantly expressed in different tissues. The highest protein levels have been observed in brain. Like its closest relative SNAP29 it lacks a membrane anchor and the mechanism which mediates the membrane localization is still under investigation.
SNAP47 co-purifies with synaptic vesicles but shows a staining pattern different from typical synaptic vesicle markers like synapsin or synaptophysin.
SNAP47 may be localized to a vesicle-pool which has not been confined to a special organelle but accumulates in the periphery of the trans-Golgi-network.